BPC-157 is supplied as a lyophilised (freeze-dried) powder in a sealed vial and is reconstituted in the laboratory before use. Done carefully, reconstitution preserves the peptide's integrity; done carelessly, it can introduce error. This is a general research-handling overview; for the compound's chemistry see our BPC-157 research guide. Everything here is for laboratory research use only.
What you'll need
- The sealed vial of lyophilised BPC-157
- A suitable solvent — bacteriostatic water is the most commonly cited choice for research handling
- A sterile syringe and needle for transferring the solvent
- An alcohol wipe for the vial septum, and a clean work area
Step 1: choose your solvent
BPC-157 is water-soluble. Bacteriostatic water is widely used because the small amount of benzyl alcohol it contains helps limit microbial growth in a multi-use vial. Plain sterile water can also dissolve the peptide but offers no such protection, so it is generally reserved for single-use preparations.
Step 2: add the solvent gently
Wipe the septum with alcohol, draw up your measured volume of solvent, and introduce it slowly down the inner wall of the vial rather than firing it directly onto the powder cake. A gentle, controlled addition reduces foaming and mechanical stress on the peptide.
Step 3: let it dissolve — don't shake
Allow the powder to dissolve on its own, swirling the vial gently if needed. Avoid vigorous shaking, which can denature or aggregate peptides and create foam. Within a short time the solution should turn clear; a cloudy or particulate solution can indicate a handling problem or degraded material.
Step 4: work out the concentration
Concentration is simply the vial's peptide mass divided by the volume of solvent added. For example, 5 mg of peptide in 2 mL of solvent gives 2.5 mg/mL. Our peptide reconstitution calculator does this for you and helps plan volumes so a given draw corresponds to the amount your protocol needs.
Step 5: store the solution correctly
Keep the lyophilised vial at -20°C until use. Once reconstituted, store the solution refrigerated at 2-8°C, protected from light, and minimise freeze-thaw cycles, which are one of the most common causes of peptide loss over time.
Common mistakes to avoid
- Shaking the vial — swirl instead; agitation damages peptides.
- Adding solvent too fast — run it down the wall to avoid foaming.
- Storing the solution at room temperature — refrigerate reconstituted material.
- Repeated freeze-thaw — aliquot before freezing if a solution will be used over time.
Frequently asked questions
BPC-157 is water-soluble and is commonly reconstituted with bacteriostatic water for research handling, as it limits microbial growth in multi-use vials.
Divide the vial's peptide mass by the solvent volume added (e.g. 5 mg in 2 mL = 2.5 mg/mL). Our peptide reconstitution calculator does this automatically.
No. Swirl the vial gently and let it dissolve; vigorous shaking can damage the peptide and cause foaming.
Refrigerated at 2-8°C, protected from light, with freeze-thaw cycles minimised. The lyophilised vial is stored at -20°C until use.
Research-grade BPC-157
Greater than 99% purity, lyophilised powder, same-day dispatch before 3PM with tracked UK & EU delivery. Research use only.
Buy BPC-157 →Disclaimer: All products supplied by PurePeptides are strictly for laboratory research use only. They are not for human or animal consumption and are not medical products. This article is informational and is not medical, clinical, or dosing advice.