For research purposes only. This guide describes the chemical identity and laboratory handling of TB-500 and summarises findings reported in published research. It is not medical advice and does not describe any effect, dose, or use in humans or animals.
What is TB-500?
TB-500 is a synthetic peptide related to Thymosin Beta-4 (Tb4), a naturally occurring 43-amino-acid peptide. It is supplied as a lyophilised powder and used as a research-grade reference compound in laboratory work.
PurePeptides offers TB-500 at >99% purity strictly for in-vitro research use. This page describes its chemical identity and handling only, and must not be interpreted as describing any effect in humans or animals.
TB-500 is a synthetic peptide associated with Thymosin Beta-4 (Tβ4), a naturally occurring 43-amino-acid actin-binding peptide. In research, Thymosin Beta-4 is studied for its role in actin sequestration, cell migration and angiogenesis. The “TB-500” name is the designation commonly used for the synthetic research-grade form. Mechanistically, the literature centres on its actin-binding behaviour, which is linked in experimental models to cytoskeletal dynamics and cell movement.
Chemical identity & specifications
| Product | TB-500 |
| Full name | Thymosin Beta-4 fragment (TB-500) |
| Synonyms | Tb4; Thymosin Beta-4 |
| Classification | Synthetic peptide (Thymosin Beta-4) |
| Amino acids | 43 (Thymosin Beta-4) |
| CAS number | 77591-33-4 |
| Solubility | Water-soluble |
| Purity | >99% |
| Physical form | Lyophilised powder |
| Storage | -20°C lyophilised; 2-8°C reconstituted |
| Intended use | Research use only - not for human consumption |
Handling TB-500 in research protocols
In laboratory settings, lyophilised TB-500 is typically stored at -20°C, protected from light and moisture, until use. For reconstitution, bacteriostatic water is a commonly cited solvent; once in solution, the material is generally kept refrigerated at 2-8°C with freeze-thaw cycles minimised to preserve peptide integrity.
Researchers quantify TB-500 by concentration within their experimental system — for example molar concentrations for in-vitro work, or amount-per-mass figures in animal models. These are parameters defined by the study design and reported in the literature; they are not dosing guidance for any human or animal use. To convert between vial mass, solvent volume, and target concentration for laboratory preparation, our peptide reconstitution calculator may be useful.
Findings reported in the research literature
Thymosin Beta-4 and TB-500 appear in in-vitro and preclinical research on actin regulation, cell migration and tissue repair. The naturally occurring parent peptide, Thymosin Beta-4, is well represented in the cell-biology literature as an actin-sequestering protein involved in cytoskeletal dynamics.
Within tissue-repair research the literature spans several model systems: dermal and muscle wound-healing models, cardiac-tissue studies, and work touching on inflammatory pathways and hair-follicle biology in experimental animals. As with related peptides, these are observations made in controlled in-vitro and animal models, used to characterise the compound rather than to demonstrate any effect in humans.
Any effects described in this literature are observations made in controlled experimental systems — cell assays and animal models — and are reported here only to summarise the scientific record. They do not constitute claims about outcomes, efficacy, benefits, or safety in humans, and nothing on this page should be read as encouraging human or animal use. Readers interested in the primary research can review the indexed literature via PubMed’s Thymosin Beta-4 results.
TB-500 and BPC-157: tissue-repair research peptides
TB-500 is frequently discussed alongside BPC-157 in tissue-repair research. Both are studied in models of cell migration, angiogenesis and connective-tissue biology, but they are chemically distinct:
| Peptide | Class | Length | Approx. MW | Origin |
| TB-500 | Synthetic Thymosin Beta-4 | 43 aa | ~4963 g/mol | Synthetic version of naturally occurring Thymosin Beta-4 |
| BPC-157 | Synthetic pentadecapeptide | 15 aa | ~1419.6 g/mol | Partial sequence from a gastric Body Protection Compound |
For the companion compound, see our BPC-157 research guide. Both are supplied by PurePeptides strictly as research materials, with no claim regarding outcomes in humans or animals.
Purity and quality control in research-grade TB-500
In laboratory research, peptide purity has a direct bearing on reproducibility: synthesis by-products or residual reagents can confound experimental observations. PurePeptides supplies TB-500 at greater than 99% purity as a lyophilised powder.
In peptide manufacturing, purity is typically assessed by reverse-phase high-performance liquid chromatography (HPLC), while identity is confirmed by mass spectrometry (MS) against the expected molecular weight — approximately 4963 g/mol for TB-500. A certificate of analysis (COA) documenting these results is available on request for the current batch. When evaluating any research-grade peptide, requesting the COA and confirming both the purity figure and the mass-spec identity is good laboratory practice.
Frequently asked questions
TB-500 is a synthetic peptide associated with Thymosin Beta-4, a naturally occurring 43-amino-acid actin-binding peptide, supplied as a lyophilised powder for research use only.
TB-500 (synthetic Thymosin Beta-4) is studied in preclinical tissue-repair research — in-vitro and animal-model work on actin regulation, cell migration and wound-healing models. It is supplied strictly as a research material, not for human or animal use.
TB-500 is the research-peptide designation for synthetic Thymosin Beta-4 (Tβ4). In the literature, Thymosin Beta-4 is studied as an actin-sequestering peptide involved in cell migration.
In published research, TB-500 dosage is expressed as a concentration or amount within the experimental system — for example molar concentrations in vitro, or amounts per kilogram in animal models. These are study-design parameters, not dosing guidance for humans.
Any effects reported for TB-500 or Thymosin Beta-4 are observations from controlled experimental systems, not statements about safety or side effects in humans. We make no claims regarding human use.
Both are studied in tissue-repair research, but they are chemically unrelated: TB-500 is a synthetic version of the 43-amino-acid Thymosin Beta-4, whereas BPC-157 is a 15-amino-acid pentadecapeptide derived from a gastric protein.
Both appear in tissue-repair research and are sometimes referenced together as distinct reference compounds. Combining them is an experimental-design decision, not a human-use protocol; neither is for human or animal use.
We supply TB-500 in the UK strictly as a research chemical for laboratory use only. It is the purchaser's responsibility to ensure compliance with the laws and regulations applicable in their jurisdiction.
PurePeptides supplies TB-500 at greater than 99% purity as a lyophilised powder. A certificate of analysis (COA) is available on request for the current batch.
Store the lyophilised powder at -20°C, protected from light and moisture. In the laboratory it is commonly reconstituted with bacteriostatic water; once in solution, store refrigerated at 2-8°C and minimise freeze-thaw cycles.
Research-grade TB-500 is available from PurePeptides at greater than 99% purity, with same-day dispatch before 3PM and tracked UK & EU delivery. It is sold strictly for laboratory research use only.
Related comparisons
Research-grade TB-500
Greater than 99% purity, lyophilised powder, same-day dispatch before 3PM with tracked UK & EU delivery. Research use only.
Buy TB-500 →Disclaimer: All products supplied by PurePeptides are strictly for laboratory research use only. They are not for human or animal consumption and are not medical products. This guide is informational and summarises publicly available research; it is not medical, clinical, or dosing advice.